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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1989-9-27
|
| pubmed:abstractText |
We studied by immunohistochemistry the features of differentiation in 24 desmoplastic and 16 classic medulloblastomas (median patient ages, 18 and 6.5 years, respectively) with the use of a panel of cytoskeletal and synaptosomal markers. A distinctive pattern of immunoreactivity with a series of monoclonal antibodies (Mabs) was documented in the polar tumor cells forming the reticulin-free pale islands of the desmoplastic variant, denoting overt neuritogenesis. These comprised the following: (1) Mab Tp-NFP1A3 recognizing an epitope in the high-molecular-weight (Mr) isoform of neurofilament protein; (2) Mab AP18 to the high-Mr microtubule-associated protein 2; (3) Mab TUJ1 recognizing the class III beta-tubulin isotype (human h beta 4); and (4) Mab SY38 to synaptophysin. Immunoblot analysis confirmed the expression of h beta 4 in three medulloblastomas, yielding strong single bands in two desmoplastic medulloblastomas and a considerably weaker band in one classic medulloblastoma. Glial fibrillary acidic protein-positive tumor cells frequently formed an integral component of the pale islands. Oligodendrogliallike areas in one classic and in three desmoplastic medulloblastomas were immunopositive for the Mabs to synaptophysin, microtubule-associated protein 2, and h beta 4, indicating a neuroblastic nature. We propose that the reticulin-free structures of desmoplastic medulloblastomas constitute neoplastic foci with features of predominantly neuronal and, to a lesser degree, astroglial differentiation.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Osteocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/Reticulin,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptophysin,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0003-9985
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
113
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1019-29
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2505732-Adolescent,
pubmed-meshheading:2505732-Adult,
pubmed-meshheading:2505732-Antibodies, Monoclonal,
pubmed-meshheading:2505732-Astrocytes,
pubmed-meshheading:2505732-Calcium-Binding Proteins,
pubmed-meshheading:2505732-Cerebellar Neoplasms,
pubmed-meshheading:2505732-Child,
pubmed-meshheading:2505732-Child, Preschool,
pubmed-meshheading:2505732-Female,
pubmed-meshheading:2505732-Humans,
pubmed-meshheading:2505732-Immunoblotting,
pubmed-meshheading:2505732-Immunohistochemistry,
pubmed-meshheading:2505732-Infant,
pubmed-meshheading:2505732-Male,
pubmed-meshheading:2505732-Medulloblastoma,
pubmed-meshheading:2505732-Membrane Proteins,
pubmed-meshheading:2505732-Neurons,
pubmed-meshheading:2505732-Osteocalcin,
pubmed-meshheading:2505732-Reticulin,
pubmed-meshheading:2505732-Synaptophysin,
pubmed-meshheading:2505732-Tubulin
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Cerebellar desmoplastic medulloblastomas. A further immunohistochemical characterization of the reticulin-free pale islands.
|
| pubmed:affiliation |
Department of Pathology, University of Virginia School of Medicine, Charlottesville 22908.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|