Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1989-9-8
|
| pubmed:abstractText |
A human acute monocytic leukemia cell line, THP-1, releases a factor which activates human cytotoxic (killer) T lymphocytes (CTL) against autologous tumor in vitro. The factor, named cytotoxic (killer) T cell activating factor (KAF), is an acidic protein of 70,000 to 100,000 dalton molecular size. Peripheral blood leukocytes from two patients, bearing epithelioid sarcoma or malignant schwannoma, were cultured for 7 days with individual autologous tumor to induce CTL directed to the corresponding tumor. Monocyte-depleted peripheral leukocytes generated lesser CTL activity than the monocyte-containing leukocyte population. However, the KAF was able to replace the monocyte function. The KAF acted at the CTL generation phase as well as the effector phase. The KAF-activated killer cells possessed CD4-8+ surface phenotype. The CTL killed autologous tumor or other unrelated tumor cell lines only when they shared some of the HLA class I antigens. It was also demonstrated that the KAF does not activate killer cells without proper antigenic stimuli, because the KAF-augmented CTL possess specificity against autologous tumor or other HLA-A or -B matched tumor cell lines. The therapeutic applicability of human KAF for anti-tumor CTL therapy against autologous tumor is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0910-5050
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
80
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
537-45
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2503475-Adult,
pubmed-meshheading:2503475-Animals,
pubmed-meshheading:2503475-Antigens, CD8,
pubmed-meshheading:2503475-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2503475-Antigens, Neoplasm,
pubmed-meshheading:2503475-Chromatography, Gel,
pubmed-meshheading:2503475-Female,
pubmed-meshheading:2503475-HLA Antigens,
pubmed-meshheading:2503475-Humans,
pubmed-meshheading:2503475-Killer Cells, Natural,
pubmed-meshheading:2503475-Leukemia, Monocytic, Acute,
pubmed-meshheading:2503475-Lymphocyte Activation,
pubmed-meshheading:2503475-Male,
pubmed-meshheading:2503475-Mice,
pubmed-meshheading:2503475-Mice, Inbred A,
pubmed-meshheading:2503475-Molecular Weight,
pubmed-meshheading:2503475-Neoplasm Proteins,
pubmed-meshheading:2503475-Neoplasms,
pubmed-meshheading:2503475-Neurilemmoma,
pubmed-meshheading:2503475-Rats,
pubmed-meshheading:2503475-Rats, Inbred Strains,
pubmed-meshheading:2503475-Sarcoma,
pubmed-meshheading:2503475-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:2503475-Tumor Cells, Cultured
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Augmentation of human cytotoxic T lymphocytes against autologous tumor by a factor released from human monocytic leukemia cell line.
|
| pubmed:affiliation |
Department of Immunology, Kochi Medical School.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|