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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1989-9-15
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pubmed:abstractText |
A HindIII gene bank of Neisseria gonorrhoeae MUG116 was constructed in the cosmid vector pHC79. A cosmid (pSY81) was isolated that was able to convert N. gonorrhoeae FA5100 to reactivity with monoclonal antibody (MAb) 2-1-L8. Several MAb-reactive transformants were isolated and characterized with respect to lipooligosaccharide (LOS) production as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, their ability to react with two other LOS-specific MAbs (3F11 and O6B4), and Southern blot analysis. Escherichia coli containing the clone had altered lipopolysaccharide expression as determined by electrophoretic analysis; however, no reactivity was seen with gonococcus-specific MAbs. The introduction of pSY81 into FA5100 had a pleiomorphic effect, giving rise to transformants having the full parental phenotype or transformants lacking reactivity to a combination of LOS-specific MAbs. Southern blot analysis indicated that the LOS biosynthetic mutation in FA5100 was not due to chromosomal rearrangement or large deletions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-2418125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-2428752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-2440807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-2492099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-2579158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3009418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3112010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3117695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3123395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3126141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3126149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3315864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-368062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-3934078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6176137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6187729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6260575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6304463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6421941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6432693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6743336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6785365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6796562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6798135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-6806436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2503447-825024
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2847-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2503447-Blotting, Southern,
pubmed-meshheading:2503447-Cloning, Molecular,
pubmed-meshheading:2503447-Escherichia coli,
pubmed-meshheading:2503447-Genes, Bacterial,
pubmed-meshheading:2503447-Genetic Vectors,
pubmed-meshheading:2503447-Lipopolysaccharides,
pubmed-meshheading:2503447-Neisseria gonorrhoeae,
pubmed-meshheading:2503447-Oligosaccharides,
pubmed-meshheading:2503447-Transformation, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Molecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.
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pubmed:affiliation |
Department of Microbiology, University of Maryland, College Park 20742.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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