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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-8-30
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pubmed:abstractText |
Pleiotropic mutations (xcp) affecting the secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at three different loci on the chromosome. The xcp-1 gene, which is located at the 0-min region, was isolated from a genomic bank containing DNA from P. aeruginosa PAO1. The recombinant cosmid pLX25 complemented the xcp::Tn5-751 insertion mutation previously described. The xcp-1 gene was located on pLX25 by mapping the insertion point of transposon Tn5-751 and by deletion and subcloning analysis. The xcp-1 gene was expressed when transcription was initiated from a tac promoter, and a 26,000-dalton protein was identified in Escherichia coli minicells. The Xcp-1 protein was associated with the membrane fraction of E. coli. A 30-kilodalton outer membrane protein was also affected by the xcp::Tn5-751 mutation in P. aeruginosa. The possible correlation between Xcp-1 and this protein is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-101518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-111024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-13851475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2503678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2536688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2821166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2842313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-2989092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3040889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3117768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3123463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3128636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3322811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-3549457,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-4605400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6094488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6201861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6281230,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6299895,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6323265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6427194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-6800952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-818504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2502537-90552
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4342-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene.
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pubmed:affiliation |
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseilles, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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