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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1989-9-1
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pubmed:abstractText |
An assay was developed for detecting beta-galactofuranosidase produced by Penicillium and Aspergillus spp. The substrate for the assay, 4-nitrophenyl beta-D-galactofuranoside, was synthesized from penta-O-acetyl-beta-D-galactofuranose and 4-nitrophenol by a tin chloride catalyzed reaction followed by O-deacetylation. Aspergillus spp. produced only small quantities of beta-galactofuranosidase during 30 d at 25 degrees C. Only the biverticillate Penicillium spp. (P. funiculosum, P. islandicum, P. rubrum and P. tardum) produced substantial beta-galactofuranosidase after 1-4 weeks at 25 degrees C. No extracellular antigens of these four Penicillium spp. could be detected in culture filtrates by the sandwich ELISA technique when antibodies to the extracellular beta-galactofuranoside-containing polysaccharide antigen of P. digitatum was used. Antigens to all other Penicillium and Aspergillus spp. were easily detected in their culture filtrates.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/exo-beta-D-galactofuranosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-8847
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-7
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pubmed:dateRevised |
2008-9-10
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pubmed:meshHeading |
pubmed-meshheading:2502527-Antigens, Fungal,
pubmed-meshheading:2502527-Aspergillus,
pubmed-meshheading:2502527-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2502527-Galactosidases,
pubmed-meshheading:2502527-Galactosides,
pubmed-meshheading:2502527-Glycoside Hydrolases,
pubmed-meshheading:2502527-Penicillium,
pubmed-meshheading:2502527-beta-Galactosidase
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pubmed:year |
1989
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pubmed:articleTitle |
Detection of beta-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl beta-D-galactofuranoside.
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pubmed:affiliation |
Purdue University, Department of Food Science, West Lafayette, Indiana 47907.
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pubmed:publicationType |
Journal Article
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