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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-8-25
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pubmed:abstractText |
Human sera were tested for the presence of anti-nef antibodies by radioimmunoassay (RIA), with recombinant radiolabelled nef expressed in E. coli. Of the 300 HIV-positive sera tested by RIA, 70 +/- 5.3% were found to be anti-nef positive. Anti-nef antibodies bound to nef with a high affinity (K 0.5 = 2.2 x 10(-9) M). In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1. The results obtained showed that the immunodominant antigenic sites of nef were located close to the N- and C-terminal regions of the molecule.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0269-9370
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-20
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2500954-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:2500954-Amino Acid Sequence,
pubmed-meshheading:2500954-Female,
pubmed-meshheading:2500954-GTP-Binding Proteins,
pubmed-meshheading:2500954-Gene Products, nef,
pubmed-meshheading:2500954-Genes, Regulator,
pubmed-meshheading:2500954-HIV,
pubmed-meshheading:2500954-HIV Antibodies,
pubmed-meshheading:2500954-Humans,
pubmed-meshheading:2500954-Male,
pubmed-meshheading:2500954-Molecular Sequence Data,
pubmed-meshheading:2500954-Peptides,
pubmed-meshheading:2500954-Recombinant Proteins,
pubmed-meshheading:2500954-Retroviridae Proteins,
pubmed-meshheading:2500954-Virus Replication,
pubmed-meshheading:2500954-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1989
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pubmed:articleTitle |
Use of synthetic peptides for the detection of antibodies against the nef regulating protein in sera of HIV-infected patients.
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pubmed:affiliation |
CNRS UA 1179-INSERM U 172, Laboratoire de Biochimie, Faculté de Médecine Nord, Marseille, France.
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pubmed:publicationType |
Journal Article
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