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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-8-4
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pubmed:abstractText |
The study was undertaken to investigate the role of dengue type 2 virus (DV)-infected mouse peritoneal macrophages (M phi) in presentation of the DV antigen to B lymphocytes as shown by counting virus-specific IgM antibody plaque-forming cells (PFC). It was observed that heat-killed or glutaraldehyde-fixed M phi did not present the antigen. Pretreatment of M phi with the lysosomotropic compounds ammonium chloride and chloroquine inhibited the antigen presentation. Depletion of M phi from the spleen cell cultures abrogated the immune response to DV. The tryptic-digested DV antigen could stimulate immune responses in B-lymphocyte enriched (depleted of M phi and T cells) spleen cell cultures, and the digested antigen could be presented by glutaraldehyde-fixed M phi. Pretreatment of M phi with a trypsin inhibitor abrogated antigen presentation. The findings thus show that even for presentation to B cells the DV antigen must be processed by M phi by a trypsin-like protease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-160396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-17815805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-3013642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-3079785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-308084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-3499385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-3873617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-4373023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-4587740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-4607056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-5390795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-562388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6193218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6228574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6458664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6644274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6795263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6798568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-6968713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-77884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2500392-82587
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-43
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2500392-Ammonium Chloride,
pubmed-meshheading:2500392-Animals,
pubmed-meshheading:2500392-Antigen-Presenting Cells,
pubmed-meshheading:2500392-Antigens, Viral,
pubmed-meshheading:2500392-B-Lymphocytes,
pubmed-meshheading:2500392-Chloroquine,
pubmed-meshheading:2500392-Dengue Virus,
pubmed-meshheading:2500392-Glutaral,
pubmed-meshheading:2500392-Hot Temperature,
pubmed-meshheading:2500392-Lymphocyte Activation,
pubmed-meshheading:2500392-Macrophages,
pubmed-meshheading:2500392-Mice,
pubmed-meshheading:2500392-Mice, Inbred Strains,
pubmed-meshheading:2500392-Trypsin Inhibitor, Kunitz Soybean
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pubmed:year |
1989
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pubmed:articleTitle |
Obligatory role of macrophages in dengue virus antigen presentation to B lymphocytes.
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pubmed:affiliation |
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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