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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1989-7-21
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pubmed:abstractText |
We describe an assay that converts the effects of tRNA-tRNA contacts at two particular codons into a quantitative effect on beta-galactosidase level. The assay measures the separate and combined efficiency of suppression at adjacent nonsense codons in vivo using a set of specially created homologous messages. In a survey of distal anticodon arm substitutions, we find that particular mutant tRNAs occupying the P-site reduce the apparent efficiency of the suppressor tRNA reading the A-site codon by factors of 2-170. By using measured tRNA-tRNA distances and the crystallographic tRNA structure, we propose a model of the tRNA-tRNA-mRNA complex. In the model, the anticodon loops of the P-site and A-site tRNAs contact one another in a way that is consistent with our combined tRNA efficiency data. These results suggest that tRNA-tRNA interactions that modulate tRNA action are an inevitable feature of translation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-2435916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-2458530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-2469803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-3270525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-3529087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-357743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-3892014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-3894009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-393826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-5444622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6178833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6188840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6188841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6195344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6345795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6374155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2499882-6753149
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4397-401
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2499882-Anticodon,
pubmed-meshheading:2499882-Base Sequence,
pubmed-meshheading:2499882-Codon,
pubmed-meshheading:2499882-Models, Molecular,
pubmed-meshheading:2499882-Molecular Sequence Data,
pubmed-meshheading:2499882-Nucleic Acid Conformation,
pubmed-meshheading:2499882-RNA, Messenger,
pubmed-meshheading:2499882-RNA, Transfer,
pubmed-meshheading:2499882-Ribosomes,
pubmed-meshheading:2499882-beta-Galactosidase
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pubmed:year |
1989
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pubmed:articleTitle |
tRNA-tRNA interactions within cellular ribosomes.
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pubmed:affiliation |
Department of Molecular, Cellular, University of Colorado, Boulder 80309-0347.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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