Linked Life Data

2498663

Source:http://linkedlifedata.com/resource/pubmed/id/2498663

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6222
pubmed:dateCreated
1989-6-27
pubmed:abstractText
Many hormones, neurotransmitters and growth factors evoke in their target cells oscillations in the free internal Ca2+ concentration [( Ca2+]i). In electrically non-excitable cells these fluctuations are due to periodic release of Ca2+ from intracellular reservoirs, stimulated by the internal messenger inositol trisphosphate (InsP3). Most models at present invoke fluctuating levels of InsP3 as a key component in generating the oscillations in [Ca2+]i. InsP3 injected into intact cells evokes irregular and transient oscillatory Ca2+-dependent current responses, but the intracellular InsP3 concentration is not constant in such experiments. Here we monitor changes in [Ca2+]i by measuring Ca2+-activated Cl- current in single internally perfused mouse pancreatic acinar cells and show that acetylcholine (ACh), acting through muscarinic receptors, evokes regular and repetitive current pulses which are mimicked by InsP3 applied through a patch pipette. To exclude the possibility that InsP3 is periodically phosphorylated or degraded, we replaced it by the non-metabolizable InsP3 analogue inositol trisphosphorothioate (InsPS3), which also evokes regular pulses of Ca2+-activated Cl- current. These effects are independent of external Ca2+, but abolished by high intracellular concentrations of a Ca2+-chelator. We conclude that repetitive pulses of intracellular Ca2+ release occur even when the concentration of InsP3 is constant.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Pulsatile intracellular calcium release does not depend on fluctuations in inositol trisphosphate concentration.
pubmed:affiliation
MRC Secretory Control Research Group, University of Liverpool, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't