Linked Life Data

2498056

Source:http://linkedlifedata.com/resource/pubmed/id/2498056

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-7-5
pubmed:abstractText
RNA molecules that differ by a single base pair in their highest melting domain can be separated by the solution melting method (Smith et al., 1988) due to sequence-specific melting properties of double-stranded (ds) RNA heteroduplexes. We now show that this method can be used to reliably detect single base pair differences in DNA molecules, and we define the upper limit of the high melting domain length that can be analyzed by this method for dsRNA and RNA-DNA molecules as about 250 bp and 130 bp, respectively. The usefulness of this method to detect point mutations in human genomic DNA is evaluated. X-linked genes are most amenable to study, because results are most easily interpretable when only a single allele is present. Using the human factor VIII gene as an example, we show this method is capable of detecting polymorphisms present in genomic DNA after amplification by the polymerase chain reaction. This technique should prove useful in the simultaneous rapid screening of multiple exons of X-linked genes for single-base mutations.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0198-0238
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Detection of single-base mutations in DNA molecules using the solution melting method.
pubmed:affiliation
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't