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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4903
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pubmed:dateCreated |
1989-6-16
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pubmed:abstractText |
The interleukin-2 receptor alpha (IL-2R alpha) chain gene contains a sequence similar to the immunoglobulin (Ig) kappa (kappa) enhancer NF-kappa B binding site. This site, which is bound by the nuclear protein, NF-kappa B, is critical for Ig kappa gene expression. The major T cell nuclear factor that binds to the IL-2R alpha site in vitro appears indistinguishable from NF-kappa B. NF-kappa B binds to IL-2R alpha and kappa sequences with similar affinities; however, only the kappa site potently activates transcription from heterologous promoters. Thus, high-affinity NF-kappa B binding in vitro cannot be equated with transcriptional activation in vivo. Mutation of the NF-kappa B binding site in the context of an IL-2 R alpha promoter construct markedly diminished promoter activity in human T cell lymphotropic virus type I (HTLV-I)-transformed MT-2 cells but not in phorbol myristate acetate-stimulated Jurkat T cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
466-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2497520-Animals,
pubmed-meshheading:2497520-Base Sequence,
pubmed-meshheading:2497520-Binding Sites,
pubmed-meshheading:2497520-Cell Line, Transformed,
pubmed-meshheading:2497520-DNA-Binding Proteins,
pubmed-meshheading:2497520-Enhancer Elements, Genetic,
pubmed-meshheading:2497520-Gene Expression Regulation,
pubmed-meshheading:2497520-HIV-1,
pubmed-meshheading:2497520-HeLa Cells,
pubmed-meshheading:2497520-Human T-lymphotropic virus 1,
pubmed-meshheading:2497520-Humans,
pubmed-meshheading:2497520-Immunoglobulin kappa-Chains,
pubmed-meshheading:2497520-Mice,
pubmed-meshheading:2497520-Molecular Sequence Data,
pubmed-meshheading:2497520-Mutation,
pubmed-meshheading:2497520-NF-kappa B,
pubmed-meshheading:2497520-Promoter Regions, Genetic,
pubmed-meshheading:2497520-Receptors, Interleukin-2,
pubmed-meshheading:2497520-T-Lymphocytes,
pubmed-meshheading:2497520-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2497520-Transcription, Genetic,
pubmed-meshheading:2497520-Transcription Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Functionally distinct NF-kappa B binding sites in the immunoglobulin kappa and IL-2 receptor alpha chain genes.
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pubmed:affiliation |
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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