Linked Life Data

2496978

Source:http://linkedlifedata.com/resource/pubmed/id/2496978

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-6-12
pubmed:abstractText
We recently demonstrated activation of 5-lipoxygenase activity in human polymorphonuclear leukocytes (PMN) on preincubation of the cells with glutathione-depleting agents, namely 1-chloro-2,4-dinitrobenzene (Dnp-C1) and azodicarboxylic acid bis[dimethylamide] (diamide). In this paper we show that Dnp-C1, but not diamide, impairs the reduction of added organic peroxides in whole PMN. Also, since co-incubation of fatty acid hydroperoxides with arachidonate caused activation of 5-lipoxygenase, we propose that Dnp-C1 increases the peroxide level in PMN which is required for the onset of lipoxygenase activity. This could be substantiated in PMN homogenates by a glutathione-dependent depression of arachidonate 5-lipoxygenation. At higher arachidonate concentrations and in the presence of Ca2+ the glutathione effect was not observed but additional glutathione peroxidase also blocked this maximally stimulated 5-lipoxygenase. Together with other experiments, it became obvious that the formation of leukotrienes, but also of 15-lipoxygenase products, requires a sharply defined threshold level of fatty acid hydroperoxides which are generated by the lipoxygenases and counteracted by glutathione-dependent peroxidase(s). Dnp-C1 influences this equilibrium by removing glutathione and thereby inhibiting glutathione-dependent peroxidase activity. From our data we conclude that it is the physiological function of the peroxidase activity in PMN to determine an efficiently regulated threshold level of hydroperoxide products, below which no activation of 5-lipoxygenase or 15-lipoxygenase can occur.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 15-Lipoxygenase, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate Lipoxygenases, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Diamide, http://linkedlifedata.com/resource/pubmed/chemical/Dinitrochlorobenzene, http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase, http://linkedlifedata.com/resource/pubmed/chemical/Leukotrienes, http://linkedlifedata.com/resource/pubmed/chemical/Peroxides
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
180
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
527-33
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Involvement of glutathione peroxidase activity in the stimulation of 5-lipoxygenase activity by glutathione-depleting agents in human polymorphonuclear leukocytes.
pubmed:affiliation
Faculty of Biology, University of Konstanz, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't