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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1989-5-31
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pubmed:abstractText |
We have determined the V region amino acid sequence and/or serologic markers (kIIIb, PSL2, and PSL3) of 24 IgM monoclonal autoantibodies with specificities of anti-gamma-globulin (RF), anti-I (cold agglutinin), anti-low density lipoprotein and anti-intermediate filaments. The data emphasize the overwhelming selection of the HumKv325/VkIIIb L chain for this family of autoantibodies. The few amino acid substitutions found within the VL regions were mainly concentrated in the complementarity-determining region 1. JK and CK genes did not show the same pattern of restriction. There is a good correlation between the amino acid sequence and the presence of the kIIIb marker. The idiotypic marker PSL2 was present in 34 out of 35 kIIIb L chains analyzed (97%) and the PSL3 in 27 (80%). Moreover, the hydrophilicity and antigenic profiles of these L chains corroborate the presence of the epitopes detected by the anti-CRI. These results demonstrate a restricted selection of the Vk genes used by a family of self reacting proteins, and an unusual evolutionary conservation of the idiotypic structure that may be involved in the network regulation.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3158-63
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2496160-Agglutinins,
pubmed-meshheading:2496160-Amino Acid Sequence,
pubmed-meshheading:2496160-Antibody Specificity,
pubmed-meshheading:2496160-Autoantibodies,
pubmed-meshheading:2496160-Cross Reactions,
pubmed-meshheading:2496160-Cryoglobulins,
pubmed-meshheading:2496160-Humans,
pubmed-meshheading:2496160-Immunoglobulin Idiotypes,
pubmed-meshheading:2496160-Immunoglobulin M,
pubmed-meshheading:2496160-Immunoglobulin kappa-Chains,
pubmed-meshheading:2496160-Intermediate Filaments,
pubmed-meshheading:2496160-Lipoproteins, LDL,
pubmed-meshheading:2496160-Molecular Sequence Data,
pubmed-meshheading:2496160-Rheumatoid Factor
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pubmed:year |
1989
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pubmed:articleTitle |
Structural and idiotypic characterization of the L chains of human IgM autoantibodies with different specificities.
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pubmed:affiliation |
Department of Pathology, New York University Medical Center 10016.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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