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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-5-25
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pubmed:abstractText |
Groups of male Wistar rats were continuously exposed to nitrogen dioxide (NO2) ranging from 1.2 to 15 ppm for 1 or 2 weeks to examine the dose-effect relationship between NO2 and the xenobiotic metabolizing activity of lung microsomes. The lung cytochrome P-450 decreased significantly after 1-week exposures to 10 and 15 ppm NO2 and showed a decreasing tendency after 2-week exposures to 6-10 ppm NO2. On the other hand, the cytochrome b5, NADPH-cytochrome P-450 reductase and NADH-cytochrome b5 reductase of lung microsomes were increased concomitant with increase in microsomal proteins during 2-week exposures to 6-10 ppm NO2. These results show that the lung cytochrome P-450 decreases preferentially upon exposure to NO2 at higher concentrations. The coumarin hydroxylase activity was the most sensitive to NO2 exposures among activities metabolizing 4 kinds of xenobiotics examined. The coumarin hydroxylase activity was decreased in a dose-dependent fashion to 67-10% of the control level by 2-week exposures to 1.2-6 ppm NO2 and became negligible at 10 ppm NO2. The 7-ethoxycoumarin O-deethylase activity was also decreased to 82-56% of the control level by 2-week exposures to 1.2-6 ppm NO2 and became a constantly reduced level at 10 ppm NO2. The benzo[a]pyrene hydroxylase activity was decreased by exposures to NO2 above 10 ppm, and the benzphetamine N-demethylase activity also decreased during 2-week exposures to 6-10 ppm NO2. These results indicate that exposures to NO2 above 1.2 ppm cause a consistent and preferential reduction in the activities of coumarin hydroxylase and 7-ethoxycoumarin O-deethylase of lung microsomes in a dose-dependent manner.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-Alkoxycoumarin O-Dealkylase,
http://linkedlifedata.com/resource/pubmed/chemical/Air Pollutants,
http://linkedlifedata.com/resource/pubmed/chemical/Aryl Hydrocarbon Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/Benzopyrene Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome Reductases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome-B(5) Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH-Ferrihemoprotein Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases, N-Demethylating,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/benzphetamine N-demethylase,
http://linkedlifedata.com/resource/pubmed/chemical/coumarin 7-hydroxylase
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0300-483X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
281-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2495581-7-Alkoxycoumarin O-Dealkylase,
pubmed-meshheading:2495581-Air Pollutants,
pubmed-meshheading:2495581-Animals,
pubmed-meshheading:2495581-Aryl Hydrocarbon Hydroxylases,
pubmed-meshheading:2495581-Benzopyrene Hydroxylase,
pubmed-meshheading:2495581-Cytochrome P-450 Enzyme System,
pubmed-meshheading:2495581-Cytochrome Reductases,
pubmed-meshheading:2495581-Cytochrome-B(5) Reductase,
pubmed-meshheading:2495581-Dose-Response Relationship, Drug,
pubmed-meshheading:2495581-Electron Transport,
pubmed-meshheading:2495581-Enzyme Induction,
pubmed-meshheading:2495581-Lung,
pubmed-meshheading:2495581-Male,
pubmed-meshheading:2495581-Microsomes,
pubmed-meshheading:2495581-Mixed Function Oxygenases,
pubmed-meshheading:2495581-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:2495581-Nitrogen Dioxide,
pubmed-meshheading:2495581-Oxidoreductases, N-Demethylating,
pubmed-meshheading:2495581-Oxygenases,
pubmed-meshheading:2495581-Rats,
pubmed-meshheading:2495581-Rats, Inbred Strains
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pubmed:year |
1989
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pubmed:articleTitle |
A selective decrease in the xenobiotic metabolizing activity of rat lungs by nitrogen dioxide exposures.
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pubmed:affiliation |
Basic Medical Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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