Linked Life Data

2495001

Source:http://linkedlifedata.com/resource/pubmed/id/2495001

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-5-11
pubmed:abstractText
The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using [gamma-35S]GTP gamma S and [gamma-32P]GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with [gamma-35S]GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1269-74
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
ras p21 and other Gn proteins are detected in mammalian cell lines by [gamma-35S]GTP gamma S binding.
pubmed:affiliation
School of Biological Sciences, University of East Anglia, Norwich, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't