| pubmed-article:2493676 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0005767 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0085820 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0021017 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0021037 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0042196 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C0724668 | lld:lifeskim |
| pubmed-article:2493676 | lifeskim:mentions | umls-concept:C1178694 | lld:lifeskim |
| pubmed-article:2493676 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2493676 | pubmed:dateCreated | 1989-4-20 | lld:pubmed |
| pubmed-article:2493676 | pubmed:abstractText | The light chain isotype of immunoglobulin-secreting blood cells was investigated by means of monolayer plaque-forming cell assays allowing direct immunofluorescence staining for cytoplasmic kappa and lambda light chains in centre cells. The study revealed that cultured, polyclonally activated pokeweed mitogen (PWM) and Epstein-Barr virus (EBV), IgM-, IgG- and IgA-secreting cells expressed the kappa light chain isotype in approximately 65% of the cells. IgM- and IgG-secreting cells induced by vaccination with pneumococcal polysaccharides had a similar percentage of kappa light chain-containing cells. In contrast, IgA-secreting cells induced by vaccination with pneumococcal polysaccharides showed a different (bimodal) distribution as regards expression of kappa light chain. The majority (56%) of the investigated individuals expressed kappa light chain in approximately 50% of the cells and the rest expressed kappa light chains in approximately 80% of the cells. The percentage of cells containing kappa light chains among spontaneous IgA-secreting cells in unvaccinated individuals was approximately 50% and thus also differed from the general pattern for mitogen-activated B cells. The light chain pattern of IgA-secreting cells from individuals vaccinated with pneumococcal polysaccharides and from unvaccinated individuals probably indicates that these cells are being derived from B-cell clones with a limited idiotypic heterogeneity, which have been selected and clonally expanded by naturally occurring antigens at the mucosal membranes. | lld:pubmed |
| pubmed-article:2493676 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2493676 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2493676 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2493676 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2493676 | pubmed:issn | 0300-9475 | lld:pubmed |
| pubmed-article:2493676 | pubmed:author | pubmed-author:HeilmannCC | lld:pubmed |
| pubmed-article:2493676 | pubmed:author | pubmed-author:BaringtonTT | lld:pubmed |
| pubmed-article:2493676 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2493676 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:2493676 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2493676 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2493676 | pubmed:pagination | 159-64 | lld:pubmed |
| pubmed-article:2493676 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:meshHeading | pubmed-meshheading:2493676-... | lld:pubmed |
| pubmed-article:2493676 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2493676 | pubmed:articleTitle | Distribution of kappa and lambda light chain isotypes among human blood immunoglobulin-secreting cells after vaccination with pneumococcal polysaccharides. | lld:pubmed |
| pubmed-article:2493676 | pubmed:affiliation | Department of Paediatrics G, University Hospital, Copenhagen, Denmark. | lld:pubmed |
| pubmed-article:2493676 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2493676 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
