Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-4-3
pubmed:abstractText
Peritoneal macrophages of Lipopolysaccharide (LPS)-refractory C3H/HeJ mouse failed to express the mRNA coding interleukin 1 (IL-1) beta when stimulated by the Ca2+ ionophore A23187 or LPS, though macrophages of LPS-responsive C3H/He responded to these stimulants. These results suggest that the defect of the response in C3H/HeJ macrophages toward LPS stimulation may be related to the Ca2+-dependent signal pathway. The extracts from the C3H/HeJ macrophages showed normal activities of both protein kinase C (PKC) and calmodulin (CaM) in comparison with those from LPS-responsive C3H/He macrophages. However, one species of CaM-binding proteins could hardly be detected by the cross-linking assay with 125I-CaM in C3H/HeJ macrophages stimulated by LPS. These results suggest that the LPS-refractory site in C3H/HeJ macrophages is related to the lack of this CaM-binding protein, and the Ca2+-dependent CaM system may play an important role in the activation of cells by LPS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
723-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:2493248-Animals, pubmed-meshheading:2493248-Ascitic Fluid, pubmed-meshheading:2493248-Calcimycin, pubmed-meshheading:2493248-Calcium, pubmed-meshheading:2493248-Calmodulin, pubmed-meshheading:2493248-Calmodulin-Binding Proteins, pubmed-meshheading:2493248-Chromatography, High Pressure Liquid, pubmed-meshheading:2493248-Egtazic Acid, pubmed-meshheading:2493248-Gene Expression Regulation, pubmed-meshheading:2493248-Interleukin-1, pubmed-meshheading:2493248-Lipopolysaccharides, pubmed-meshheading:2493248-Macrophage Activation, pubmed-meshheading:2493248-Macrophages, pubmed-meshheading:2493248-Mice, pubmed-meshheading:2493248-Mice, Inbred C3H, pubmed-meshheading:2493248-Mice, Mutant Strains, pubmed-meshheading:2493248-Protein Kinase C, pubmed-meshheading:2493248-RNA, Messenger, pubmed-meshheading:2493248-Receptors, Fc
pubmed:year
1989
pubmed:articleTitle
Defect of calmodulin-binding protein in expression of interleukin-1 beta gene by LPS-nonresponder C3H/HeJ mouse macrophages.
pubmed:affiliation
Department of Microbiology, Jichi Medical School, Tochigi-ken, Japan.
pubmed:publicationType
Journal Article