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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-3-1
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pubmed:abstractText |
Nonproductively rearranged H and L chain loci of B cell hybridoma lines expressing heavily mutated antibodies were cloned and partially sequenced. The results confirm earlier data showing that somatic point mutations are as frequent in nonproductively rearranged loci containing a rearranged V gene as in productively rearranged loci. They establish in addition that in nonproductive H chain loci which bear a DJH rearrangement the frequency of somatic mutations is more than 10 times lower (0.2%) than in VDJH loci expressed by the same cells (2.5%). Thus, the hypermutation mechanism operating in B cell differentiation is targeted at V genes rearranged to the J locus and may require nucleotide sequences associated with both V and J elements in order to be fully activated. An inversion of the JH2 segment was detected in one DJH locus. This inversion appears to be the result of a secondary joining event occurring occasionally in the course of B cell development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1022-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2492327-Animals,
pubmed-meshheading:2492327-B-Lymphocytes,
pubmed-meshheading:2492327-Base Sequence,
pubmed-meshheading:2492327-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:2492327-Gene Rearrangement, B-Lymphocyte, Light Chain,
pubmed-meshheading:2492327-Genes, Immunoglobulin,
pubmed-meshheading:2492327-Hybridomas,
pubmed-meshheading:2492327-Immunoglobulin Heavy Chains,
pubmed-meshheading:2492327-Immunoglobulin Joining Region,
pubmed-meshheading:2492327-Immunoglobulin Variable Region,
pubmed-meshheading:2492327-Immunoglobulin kappa-Chains,
pubmed-meshheading:2492327-Lymphocyte Activation,
pubmed-meshheading:2492327-Mice,
pubmed-meshheading:2492327-Mice, Inbred BALB C,
pubmed-meshheading:2492327-Mice, Inbred C57BL,
pubmed-meshheading:2492327-Mutation
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pubmed:year |
1989
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pubmed:articleTitle |
V gene rearrangement is required to fully activate the hypermutation mechanism in B cells.
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pubmed:affiliation |
Institute for Genetics, University of Cologne, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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