Linked Life Data

2491751

Source:http://linkedlifedata.com/resource/pubmed/id/2491751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-1-25
pubmed:abstractText
The role of epidermal growth factor in the regulation of the proteolytic and RBC cytolytic activity of the A431 cancer cell line has been evaluated using our previously described gelatin/polyacrylamide electrophoretic assay and tumor-induced RBC cytolysis assay, respectively. A431 cells maintained in 10% fetal bovine serum were actively cytolytic for RBC (release index, 53.0 +/- 2.9%), whereas serum-starved cells maintained in serum-free medium were not cytolytic for RBC. RBC cytotoxicity was restored by adding as little as 3.4 pM epidermal growth factor to the serum-deprived cells. The RBC cytolytic stimulating activity of epidermal growth factor could be mimicked by the metal chelating agent 1,10-phenanthroline, suggesting a possible role for calcium ions in the action of epidermal growth factor and proteases. An enriched cell membrane preparation of A431 cells was also cytolytic for RBC but was unaffected by metal chelating agents. RBC-induced cytotoxicity was inhibited by the protease inhibitor leupeptin. Gelatin substrate gels of enriched A431 cell membrane preparations and serum-free supernatants revealed a pattern of high- and low-molecular-weight proteases that were stimulated by metal chelators and inhibited by leupeptin. The activity of these proteases appears to be regulated by epidermal growth factor by a process that may involve divalent cations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-84
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Role of epidermal growth factor in the expression of A431 cancer cell protease and red blood cell cytotoxicity.
pubmed:affiliation
Department of Research, Veterans Administration Medical Center, Northport, New York 11768.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.