Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-2-28
pubmed:abstractText
The effect of ethanol on the fractionation of gliadin by cellulose-based ion exchange column chromatography was studied. Ten peaks have been separated on DEAE- and 12 peaks on CM-cellulose column using a complex elution technique. By SDS-polyacrylamide gel electrophoresis of the separated fractions, it was observed that ethanol incorporated into the eluent improved the resolution of some fractions. Some of the peaks gave only 2 or 3 protein bands in electrophoregrams. Since ethanol does not affect the reproducibility in the two types of chromatographic medium, the method seems to be suitable for purification of gliadin fractions in large quantities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0237-6261
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-90
pubmed:dateRevised
2002-11-1
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
The effect of ethanol in the separation of wheat gliadins by CM- and DEAE-cellulose chromatography.
pubmed:affiliation
Department of Plant Physiology, Eötvös Loránd University, Budapest, Hungary.
pubmed:publicationType
Journal Article