Linked Life Data

2482404

Source:http://linkedlifedata.com/resource/pubmed/id/2482404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-3-9
pubmed:abstractText
The synthesis of alpha-fetoprotein in primary cultures of adult mouse hepatocytes was studied under different conditions of cultivation. On dried collagen, the majority of cells resumed synthesis of alpha-fetoprotein. Alpha-fetoprotein re-expression was accompanied by the flattening of hepatocytes, loss of gap junctional communication and domain-specific membrane antigens, and by alteration of actin pattern. Cultivation of hepatocytes on polymerized collagen gel did not prevent the re-expression of alpha-fetoprotein and other alterations described above. Hepatocyte cultivation within three-dimensional collagen gel as well as co-cultivation of hepatocytes with liver epithelial cells IAR-20 led to re-establishment of organotypic trabeculae-like structures consisting of polygonal hepatocytes with normal pattern of actin, membrane antigens and cell-cell communication. These structures were almost alpha-fetoprotein-negative. It is suggested that hepatocyte shape is co-ordinately regulated with the switch from an adult "block" of hepatocyte functions to an embryonic one. Three-dimensional extracellular matrix may in turn, influence the maintenance of hepatocyte shape.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0735-1313
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-107
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Synthesis of alpha-fetoprotein in hepatocytes is co-ordinately regulated with cell-cell and cell-matrix interactions.
pubmed:affiliation
All-Union Cancer Research Centre, Moscow, USSR.
pubmed:publicationType
Journal Article