Linked Life Data

2480959

Source:http://linkedlifedata.com/resource/pubmed/id/2480959

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
1990-2-1
pubmed:databankReference
pubmed:abstractText
We report the isolating and sequencing of three cDNA clones encoding rat P-450scc, the nucleotide and protein sequences of which are highly homologous to those of bovine and human P-450scc, especially in the putative heme and steroid binding domains. We document that different molecular mechanisms regulate P-450scc in granulosa cells of preovulatory (PO) follicles prior to and after luteinization. Luteinizing hormone/human chorionic gonadotropin (LH/hCG) and cAMP are obligatory to induce P-450scc mRNA in PO granulosa cells in vivo and in vitro. Once P-450scc mRNA is induced as a consequence of the LH/hCG surge it is constitutively maintained by luteinized cells in vivo (0-4 days) and in vitro (0-9 days) in the absence of gonadotropins, is susceptible to modulation by prolactin and is no longer regulated by cAMP. Exposure to elevated concentrations of hCG in vivo for 5-7 h was required for PO granulosa cells to undergo a functional transition establishing the stable luteal cell phenotype. Transient exposure of PO + hCG (7 h) follicles in vitro to the RNA synthesis inhibitor actinomycin D (1 microgram/ml) or the protein synthesis inhibitor cycloheximide (10 micrograms/ml), for 1-5 h prior to culturing the granulosa cells failed to disrupt the induction of P-450scc mRNA, progesterone biosynthesis, and appearance of the luteal cell morphology. Inhibitors of protein kinase A (Rp-cAMPS; 1-500 microM and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H8); 1-200 microM) added directly to the luteinized cell cultures also failed to alter P-450scc mRNA in these cells, although the cells contain in vivo amounts of mRNA for RII beta, RI alpha, and C alpha, the primary subunits of protein kinase A found in the rat ovary. These data suggest that expression of the P-450scc gene in rat ovarian follicular cells is regulated in a sequential manner by cAMP-dependent and cAMP-independent mechanisms associated with granulosa cells and luteal cells, respectively.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21934-42
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:2480959-Amino Acid Sequence, pubmed-meshheading:2480959-Animals, pubmed-meshheading:2480959-Base Sequence, pubmed-meshheading:2480959-Blotting, Northern, pubmed-meshheading:2480959-Cattle, pubmed-meshheading:2480959-Cells, Cultured, pubmed-meshheading:2480959-Cholesterol Side-Chain Cleavage Enzyme, pubmed-meshheading:2480959-Chorionic Gonadotropin, pubmed-meshheading:2480959-Cloning, Molecular, pubmed-meshheading:2480959-Corpus Luteum, pubmed-meshheading:2480959-Cyclic AMP, pubmed-meshheading:2480959-DNA, pubmed-meshheading:2480959-Female, pubmed-meshheading:2480959-Gene Expression Regulation, Enzymologic, pubmed-meshheading:2480959-Gene Library, pubmed-meshheading:2480959-Granulosa Cells, pubmed-meshheading:2480959-Humans, pubmed-meshheading:2480959-Kinetics, pubmed-meshheading:2480959-Molecular Sequence Data, pubmed-meshheading:2480959-Progesterone, pubmed-meshheading:2480959-Prolactin, pubmed-meshheading:2480959-Protein Kinases, pubmed-meshheading:2480959-RNA, pubmed-meshheading:2480959-RNA, Messenger, pubmed-meshheading:2480959-Rats, pubmed-meshheading:2480959-Restriction Mapping, pubmed-meshheading:2480959-Sequence Homology, Nucleic Acid
pubmed:year
1989
pubmed:articleTitle
Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc.
pubmed:affiliation
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.