Linked Life Data

2480903

Source:http://linkedlifedata.com/resource/pubmed/id/2480903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1990-1-30
pubmed:abstractText
Previously we have shown that a high proportion (5%-15%) of peritoneal Ly-1+ B cells that bind the common membrane phospholipid, phosphatidylcholine, express either of two VH genes. One of these genes, CH34VH, belongs to the recently described VH11 family, whereas the other VH gene, CH27VH, is reported here to belong to a new, single-member family which we designate VH12. We have characterized these new VH families in terms of strain polymorphism, map position relative to nine other VH gene families, and frequency of expression in polyclonally stimulated splenic B cell populations. We find that VH11 and VH12 genes are expressed at frequencies similar to the VH genes of other families in the spleen, and that these genes are not located among the J-proximal VH segments which are preferentially expressed during early development. These results suggest that the VH11 and VH12 genes are not preferentially rearranged, and support our earlier studies which suggested that the high frequency of VH11 and VH12 expression among peritoneal Ly-1 B cells is due to antigen selection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2115-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2480903-Animals, pubmed-meshheading:2480903-Antigens, CD5, pubmed-meshheading:2480903-Antigens, Differentiation, pubmed-meshheading:2480903-Antigens, Ly, pubmed-meshheading:2480903-B-Lymphocytes, pubmed-meshheading:2480903-Base Sequence, pubmed-meshheading:2480903-Blotting, Southern, pubmed-meshheading:2480903-Chromosome Mapping, pubmed-meshheading:2480903-Gene Rearrangement, B-Lymphocyte, Heavy Chain, pubmed-meshheading:2480903-Genes, Immunoglobulin, pubmed-meshheading:2480903-Immunoglobulin Heavy Chains, pubmed-meshheading:2480903-Immunoglobulin Variable Region, pubmed-meshheading:2480903-Mice, pubmed-meshheading:2480903-Molecular Sequence Data, pubmed-meshheading:2480903-Multigene Family, pubmed-meshheading:2480903-Polymorphism, Restriction Fragment Length, pubmed-meshheading:2480903-Spleen
pubmed:year
1989
pubmed:articleTitle
Organization and expression of VH gene families preferentially expressed by Ly-1+ (CD5) B cells.
pubmed:affiliation
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't