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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1990-1-19
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pubmed:abstractText |
We have established an Ag-specific in vitro system for studying the roles of Ag and IL-5 in B cell differentiation and Ig production. The murine B cell leukemia, BCL1B1, was transfected with mu and kappa genomic sequences and VS107 V regions that conferred a T15 Id and phosphocholine-binding specificity upon the cells. Transfected cells were treated with both the T-dependent Ag phosphorylcholine-key-hole limpet hemocyanin (PC-KLH), and 0.5 ng/ml purified IL-5. After 3.5 days steady state mu-mRNA levels increased three- to fourfold over mu-mRNA levels obtained from untreated cultures, or cultures treated with IL-5 or PC-KLH alone. IL-5 alone caused increased Ig secretion and a nearly twofold increase in proliferation. Additionally, cells treated with PC-KLH alone were able to process the Ag and present it to T cells as shown by subsequent lymphokine production. Thus, both Ag and IL-5 used singly appear to interact with their respective receptors, although neither Ag nor IL-5 increased mu-mRNA levels when used singly. The production of increased mu-mRNA levels obtained with Ag plus IL-5 required polymerase II transcription, suggesting that they may act to increase Ig transcription directly.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Hemocyanin,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin mu-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/keyhole-limpet hemocyanin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3934-9
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:2480380-Animals,
pubmed-meshheading:2480380-Antibody Specificity,
pubmed-meshheading:2480380-B-Lymphocytes,
pubmed-meshheading:2480380-Cell Line,
pubmed-meshheading:2480380-Epitopes,
pubmed-meshheading:2480380-Gene Expression Regulation,
pubmed-meshheading:2480380-Genes, Immunoglobulin,
pubmed-meshheading:2480380-Haptens,
pubmed-meshheading:2480380-Hemocyanin,
pubmed-meshheading:2480380-Immunoglobulin mu-Chains,
pubmed-meshheading:2480380-Interleukin-5,
pubmed-meshheading:2480380-Lymphocyte Activation,
pubmed-meshheading:2480380-Mice,
pubmed-meshheading:2480380-Phosphorylcholine,
pubmed-meshheading:2480380-RNA, Messenger,
pubmed-meshheading:2480380-Transcription, Genetic,
pubmed-meshheading:2480380-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
Induction of immunoglobulin mu mRNA in a B cell transfectant stimulated with interleukin-5 and a T-dependent antigen.
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pubmed:affiliation |
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
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pubmed:publicationType |
Journal Article
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