|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1989-12-28
|
|
pubmed:abstractText |
The promoter activity of a cloned inverted terminal repeat (ITR) of human adenovirus (Ad) type 5 was found to be oriented in the opposite direction with respect to that of DNA replication in a cell-free transcription system using HeLa nuclear extracts. The major transcript was initiated outside the Ad sequence about 30 nucleotides downstream from the putative TATA-box located between nucleotide positions 9-18 of Ad 5 left terminus. Competitive transcription experiments using double-stranded oligonucleotides and isolated nuclear factor I revealed that nuclear factor I and its cognate binding site located upstream of the putative TATA-box are involved in negative regulation of the transcription from ITR promoter.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
164
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1212-9
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:2480114-Adenoviruses, Human,
pubmed-meshheading:2480114-Base Sequence,
pubmed-meshheading:2480114-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:2480114-Cloning, Molecular,
pubmed-meshheading:2480114-DNA, Viral,
pubmed-meshheading:2480114-DNA-Binding Proteins,
pubmed-meshheading:2480114-HeLa Cells,
pubmed-meshheading:2480114-Humans,
pubmed-meshheading:2480114-Molecular Sequence Data,
pubmed-meshheading:2480114-NFI Transcription Factors,
pubmed-meshheading:2480114-Nuclear Proteins,
pubmed-meshheading:2480114-Oligonucleotide Probes,
pubmed-meshheading:2480114-Promoter Regions, Genetic,
pubmed-meshheading:2480114-RNA-Directed DNA Polymerase,
pubmed-meshheading:2480114-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2480114-Restriction Mapping,
pubmed-meshheading:2480114-Transcription, Genetic,
pubmed-meshheading:2480114-Transcription Factors,
pubmed-meshheading:2480114-Y-Box-Binding Protein 1
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Nuclear factor I represses the reverse-oriented transcription from the adenovirus type 5 DNA terminus.
|
|
pubmed:affiliation |
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
