rdf:type |
|
lifeskim:mentions |
umls-concept:C0005575,
umls-concept:C0008633,
umls-concept:C0031678,
umls-concept:C0162788,
umls-concept:C0180860,
umls-concept:C0439202,
umls-concept:C0475264,
umls-concept:C0581406,
umls-concept:C1441672,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1704449,
umls-concept:C1704684,
umls-concept:C1710082,
umls-concept:C1979874,
umls-concept:C2349975
|
pubmed:issue |
3
|
pubmed:dateCreated |
1990-1-10
|
pubmed:abstractText |
In situ hybridization with DNA probes labeled with biotin and detected by the avidin-alkaline phosphatase/5-bromo-chloroindoxyl phosphate-nitro blue tetrazolium system has been used to localize DNA sequences in chromosomes. To observe the hybridization signals, a phase contrast microscope has often been used because of the good visibility it provides. Use of a 595 nm band pass filter with the phase contrast microscope enhances signal contrast after in situ hybridization without reducing resolution.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0038-9153
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
64
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
139-42
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2480004-Alkaline Phosphatase,
pubmed-meshheading:2480004-Animals,
pubmed-meshheading:2480004-Avidin,
pubmed-meshheading:2480004-Biotin,
pubmed-meshheading:2480004-Cell Transformation, Viral,
pubmed-meshheading:2480004-Chromosomes,
pubmed-meshheading:2480004-DNA, Viral,
pubmed-meshheading:2480004-DNA Probes,
pubmed-meshheading:2480004-Microscopy, Phase-Contrast,
pubmed-meshheading:2480004-Nucleic Acid Hybridization,
pubmed-meshheading:2480004-Staining and Labeling
|
pubmed:year |
1989
|
pubmed:articleTitle |
A 595 nanometer band-pass filter enhances the contrast of in situ hybridization signals on chromosome observed after biotin/avidin-alkaline phosphatase localization.
|
pubmed:affiliation |
Department of Animal Breeding and Genetics, National Institute of Animal Industry, Ibaraki, Japan.
|
pubmed:publicationType |
Journal Article
|