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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-12-28
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pubmed:abstractText |
The binding activity of several galanin fragments and analogs was measured on specific receptors present in rat brain and the rat pancreatic beta cell line Rin m 5F. In both tissues it was observed that: 1) galanin(3-29), galanin(10-29) and [Ile2]-galanin were ineffective for inhibiting [125I] galanin binding and 2) active peptides had the following rank order of potency: galanin(1-29) greater than [Ac-Trp2]-galanin(2-29) greater than galanin(2-29) greater than galanin(1-15) greater than [Phe2]-galanin greater than [Tyr2]-galanin. It was concluded that the N-terminal portion of galanin is very important for interaction with central or peripheral receptors. The aromatic amino acid in position 2 (Trp in native galanin) plays a crucial role.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0196-9781
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
757-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2479930-Amino Acids,
pubmed-meshheading:2479930-Animals,
pubmed-meshheading:2479930-Binding, Competitive,
pubmed-meshheading:2479930-Brain Chemistry,
pubmed-meshheading:2479930-Cell Line,
pubmed-meshheading:2479930-Cells, Cultured,
pubmed-meshheading:2479930-Galanin,
pubmed-meshheading:2479930-Iodine Radioisotopes,
pubmed-meshheading:2479930-Islets of Langerhans,
pubmed-meshheading:2479930-Male,
pubmed-meshheading:2479930-Membranes,
pubmed-meshheading:2479930-Nerve Tissue Proteins,
pubmed-meshheading:2479930-Peptide Fragments,
pubmed-meshheading:2479930-Peptides,
pubmed-meshheading:2479930-Rats,
pubmed-meshheading:2479930-Rats, Inbred Strains,
pubmed-meshheading:2479930-Receptors, Galanin,
pubmed-meshheading:2479930-Receptors, Gastrointestinal Hormone,
pubmed-meshheading:2479930-Structure-Activity Relationship
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pubmed:articleTitle |
Structural requirements for galanin interaction with receptors from pancreatic beta cells and from brain tissue of the rat.
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pubmed:affiliation |
INSERM U178, Unité de Recherches sur la Différenciation et la Neuroendocrinologie de Cellules Digestives, Bâtiment INSERM, Villejuif, France.
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pubmed:publicationType |
Journal Article,
In Vitro
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