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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1978-8-14
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pubmed:abstractText |
The sedimentation and electrophoretic properties of Syrian hamster cytosolix and mitochondrial methionyl- and leucyl- +RNAs have been compared under denaturing conditions. Mitochondrial leucyl-tRNA could be separated into three species by chromatography on RPC-5. Their apparent molecule weights as determined by polyacrylamide slab gel elecltrophoresis were 23 000 for one species and 24 000 for the other two compared to the five cytosolic leucyl-tRNA species whose apparent molecular weights ranged from 26 000 to 28 000. Mitochondrial leucyl-tRNAs sedimented more slowly than their cytosolic counterparts, again indicating a lower molecular weight. The apparent molecular weights of the mitochondrial methionyl-tRNAs were identical or only slightly lower than their cytosolic counterparts as determined by polyacrylamide slab gel electrophoresis but both mitochondrial methionyl-tRNA and formylmethionyl-tRNA sedimented slightly more slowly than cytolsolic methionyl-tRNA. It is suggested that mitochondrial tRNAs fall into the size range of other t RNAs and might be uniform in size.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrous Acid,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Amino Acyl
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
518
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
308-20
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:247992-Animals,
pubmed-meshheading:247992-Cricetinae,
pubmed-meshheading:247992-Cytosol,
pubmed-meshheading:247992-Leucine,
pubmed-meshheading:247992-Methionine,
pubmed-meshheading:247992-Mitochondria, Liver,
pubmed-meshheading:247992-Molecular Weight,
pubmed-meshheading:247992-N-Formylmethionine,
pubmed-meshheading:247992-Nitrous Acid,
pubmed-meshheading:247992-RNA, Transfer, Amino Acyl
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pubmed:year |
1978
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pubmed:articleTitle |
Chemical and physical properties of mammalian mitochondrial aminoacyl-transfer RNAs. I. Molecular weights of mitochondrial leucyl- and methionyl-transfer RNAs.
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pubmed:publicationType |
Journal Article
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