Linked Life Data

2478712

Source:http://linkedlifedata.com/resource/pubmed/id/2478712

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-12-5
pubmed:abstractText
In Saccharomyces cerevisiae the function of the RN A1 gene is believed to be required for the transport of newly synthesized mRNA from the nucleus to the cytoplasm. Nuclear poly(A)+ RNAs accumulate and cytoplasmic mRNAs decay after the temperature-sensitive (ts) rna1.1 mutant is shifted from 25 degrees C to 37 degrees C. In this study the 3' poly(A) upon poly(A)+ RNA synthesized after expression of rna1.1 was shown to be appreciably longer than the poly(A) normally present on yeast cytoplasmic mRNA. This increased poly(A) length is due to rna1.1, since it was found only in this mutant after a 25 degrees C to 37 degrees C heat shock, not an intragenic non-ts revertant of rna1.1, wild-type (RN A1+) cells or a RN A1+, rna2.1 mutant subjected to equivalent heat shocks. It may be an indication that the normal shortening of the poly(A) on mRNAs does not occur in the nucleus, but happens only with transport to the cytoplasm. Alterations in the mean size of poly(A) may be a relatively simple marker for mRNA transport defects.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
208
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
697-700
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Yeast mutation thought to arrest mRNA transport markedly increases the length of the 3' poly(A) on polyadenylated RNA.
pubmed:affiliation
Department of Biochemistry, University College London, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't