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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
31
|
| pubmed:dateCreated |
1989-11-28
|
| pubmed:abstractText |
Plastid protein coding regions in plants are generally flanked by 3' inverted repeat (IR) sequences. In a previous work (Stern, D. B., and Gruissem, W. (1987) Cell 51, 1145-1157), we have shown that their role may be in RNA stabilization and as a processing signal that establishes the mature mRNA 3' end. In this report we have investigated the stability and protein interaction of chloroplast mRNA 3' IR-RNA sequences in more detail. Progressive deletions into the 3' IR-RNA sequences for the chloroplast cytochrome b6/f subunit IV (petD) mRNA reduce the stability of the RNA, indicating that the potential to form a stem/loop is a minimum requirement for petD 3' IR-RNA stability in vitro. Specific point mutants also destabilize the processed 3' IR-RNA, suggesting an important role for the primary sequence. Gel mobility shift and UV-cross-linking analysis has shown that 3' IR-RNAs of petD and two other chloroplast mRNAs (rbcL and psbA) interact with proteins in vitro. Comparison of the bound petD 3' IR-RNA proteins with proteins that bind to rbcL and psbA reveals that binding of certain proteins is gene-specific. Also, precursor and processed petD 3' IR-RNAs bind different sets of proteins. A single nucleotide transversion (T----A) near the base of the stem eliminates the binding of a 29-kDa protein to the petD 3' IR-RNA precursor. We discuss the possible role of 3' IR-RNA-protein interactions in plastid mRNA 3' end maturation and differential mRNA stability.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome b Group,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome b6f Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
18742-50
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2478550-Base Sequence,
pubmed-meshheading:2478550-Binding, Competitive,
pubmed-meshheading:2478550-Carrier Proteins,
pubmed-meshheading:2478550-Chloroplasts,
pubmed-meshheading:2478550-Chromatography,
pubmed-meshheading:2478550-Cytochrome b Group,
pubmed-meshheading:2478550-Cytochrome b6f Complex,
pubmed-meshheading:2478550-Drug Stability,
pubmed-meshheading:2478550-Molecular Sequence Data,
pubmed-meshheading:2478550-Mutation,
pubmed-meshheading:2478550-Nucleic Acid Conformation,
pubmed-meshheading:2478550-Plant Proteins,
pubmed-meshheading:2478550-Plants,
pubmed-meshheading:2478550-RNA,
pubmed-meshheading:2478550-RNA, Messenger,
pubmed-meshheading:2478550-RNA Precursors,
pubmed-meshheading:2478550-RNA-Binding Proteins,
pubmed-meshheading:2478550-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2478550-Ultraviolet Rays
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Function of plastid mRNA 3' inverted repeats. RNA stabilization and gene-specific protein binding.
|
| pubmed:affiliation |
Department of Plant Biology, University of California, Berkeley 94720.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|