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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-12-4
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pubmed:databankReference |
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pubmed:abstractText |
A structural and functional model of tenascin was elaborated using recombinant parts of three alternatively spliced tenascin variants and anti-tenascin monoclonal antibodies. The fusion proteins were compared with intact tenascin for their functions and by electron microscopy. A strong cell binding site was localized within 104 amino acids. This fragment also contains the epitope of the monoclonal antibody anti-Tn68, which inhibits cell attachment to tenascin and binds near the tips of the six arms of tenascin. In contrast, constructs containing the 13 1/2 EGF-like repeats of tenascin showed an antiadhesive effect. The coexistence of the two contrary signals on the same molecule might be responsible for the versatile features of tenascin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
325-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2478295-Amino Acid Sequence,
pubmed-meshheading:2478295-Animals,
pubmed-meshheading:2478295-Binding Sites,
pubmed-meshheading:2478295-Cell Adhesion,
pubmed-meshheading:2478295-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:2478295-Chick Embryo,
pubmed-meshheading:2478295-DNA,
pubmed-meshheading:2478295-Gene Library,
pubmed-meshheading:2478295-Information Systems,
pubmed-meshheading:2478295-L Cells (Cell Line),
pubmed-meshheading:2478295-Microscopy, Electron,
pubmed-meshheading:2478295-Molecular Sequence Data,
pubmed-meshheading:2478295-Nerve Tissue Proteins,
pubmed-meshheading:2478295-Peptide Fragments,
pubmed-meshheading:2478295-Protein Conformation,
pubmed-meshheading:2478295-Recombinant Fusion Proteins,
pubmed-meshheading:2478295-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2478295-Skin,
pubmed-meshheading:2478295-Tenascin
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pubmed:year |
1989
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pubmed:articleTitle |
Two contrary functions of tenascin: dissection of the active sites by recombinant tenascin fragments.
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pubmed:affiliation |
Friedrich Miescher-Institut, Basel, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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