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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-11-21
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pubmed:abstractText |
The c-src protein isolated from neuronal cells (pp60c-src+) displays a higher level of protein kinase activity than does pp60c-src from nonneural tissues. There are two structural alterations present in the amino-terminal half of pp60c-src+ expressed in neurons which could contribute to the enhanced activity of this form of pp60c-src: (i) a hexapeptide insert located at amino acid 114 of avian pp60c-src+ and (ii) a novel site(s) of serine phosphorylation. We characterized pp60c-src+ expressed in a nonneuronal cell type to identify factors that regulate the activity of the c-src+ protein and the importance of the neuronal environment on this regulation. The c-src+ protein overexpressed in chicken embryo fibroblasts (CEFs) displayed higher kinase activity than did pp60c-src. The major sites of phosphorylation of the c-src+ protein were Ser-17 and Tyr-527. The unique site(s) of serine phosphorylation originally identified in pp60c-src+ expressed in neurons was not detected in the c-src+ protein overexpressed in CEFs. Therefore, the hexapeptide insert is sufficient to cause an elevation in the tyrosine protein kinase activity of pp60c-src+. Our data also indicate that CEFs infected with the Rous sarcoma virus (RSV)c-src+ display phenotypic changes that distinguish them from cultures producing pp60c-src and that pp60c-src+-expressing cells are better able to grow in an anchorage-independent manner. The level of total cellular tyrosine phosphorylation in RSVc-src+-infected cultures was moderately higher than the level observed in cultures infected with RSVc-src. This level was not as pronounced as that observed in cells infected with RSVv-src or oncogenic variants of RSVc-src. Thus, pp60c-src+ could be considered a partially activated c-src variant protein much like other c-src proteins that contain mutations in the amino-terminal domain.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-214578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2412121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2414774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2419901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2420005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2424022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2425362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2432397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2439897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2440106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2445628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2448604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2450282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2452398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2453056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2457151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2462704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2463469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2465527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2471059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2580441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2582236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2822389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2830484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2831461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-2940239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3011421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3023673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3095923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3103925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3103926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3103927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3125343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3511908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-3920510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6086011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6087323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6100962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6159984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6192323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6194425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6198092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6201361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6207061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6254669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6299580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6312092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6314123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6323166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2477684-6428952
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3332-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2477684-Animals,
pubmed-meshheading:2477684-Brain,
pubmed-meshheading:2477684-Phosphorylation,
pubmed-meshheading:2477684-Chick Embryo,
pubmed-meshheading:2477684-Fibroblasts,
pubmed-meshheading:2477684-Cells, Cultured,
pubmed-meshheading:2477684-Phosphopeptides,
pubmed-meshheading:2477684-Nerve Tissue Proteins,
pubmed-meshheading:2477684-Phenotype,
pubmed-meshheading:2477684-Plasmids,
pubmed-meshheading:2477684-Recombinant Proteins,
pubmed-meshheading:2477684-Phosphoserine
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