| pubmed-article:2477509 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2477509 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2477509 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2477509 | lifeskim:mentions | umls-concept:C0555198 | lld:lifeskim |
| pubmed-article:2477509 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:2477509 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:2477509 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2477509 | pubmed:dateCreated | 1989-11-17 | lld:pubmed |
| pubmed-article:2477509 | pubmed:abstractText | A human malignant glioma cell line, U-251 Mg, cultured under serum free conditions, was shown to produce a growth factor for BALB/c 3T3 cells (glioma-derived growth factor-1, GDGF-1). The biological activity of GDGF-1 resided in a heat- and acid-resistant protein with a molecular weight (MW) of 25 kDa estimated by gel permeation chromatography. GDGF-1 activity was neutralized by a goat anti-human platelet derived growth factor (PDGF) antibody, indicating that the two factors were immunologically related. Furthermore, U-251 Mg cells constitutively expressed c-sis mRNA. When U-251 Mg cells were stimulated with bacterial lipopolysaccharide, 2 novel growth factors (GDGF-2 and GDGF-3) were produced in addition to the PDGF-like substance. GDGF-2 was determined to be greater than 100 kDa MW and was not neutralized by the goat anti-PDGF antiserum. The biological activity of GDGF-3 was also heat- and acid-resistant with an apparent 14 kDa MW. This factor also did not show any common antigenicity with PDGF. GDGF-2 and GDGF-3 are currently under investigation and evidence as to their natures will be published elsewhere. Our findings with this glioma cell line provide further evidence that inappropriate expression of growth factor-related genes could play important autocrine role(s) in the processes leading to malignant transformation and/or uncontrolled proliferation and may provide a paracrine stimulus for such processes as glioma neovascularization. | lld:pubmed |
| pubmed-article:2477509 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2477509 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477509 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2477509 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2477509 | pubmed:issn | 0167-594X | lld:pubmed |
| pubmed-article:2477509 | pubmed:author | pubmed-author:EstesJ EJE | lld:pubmed |
| pubmed-article:2477509 | pubmed:author | pubmed-author:GillespieG... | lld:pubmed |
| pubmed-article:2477509 | pubmed:author | pubmed-author:YokotaSS | lld:pubmed |
| pubmed-article:2477509 | pubmed:author | pubmed-author:MahaleyM... | lld:pubmed |
| pubmed-article:2477509 | pubmed:author | pubmed-author:KuratsuJJ | lld:pubmed |
| pubmed-article:2477509 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2477509 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:2477509 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2477509 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2477509 | pubmed:pagination | 225-35 | lld:pubmed |
| pubmed-article:2477509 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:meshHeading | pubmed-meshheading:2477509-... | lld:pubmed |
| pubmed-article:2477509 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2477509 | pubmed:articleTitle | Growth factors derived from a human malignant glioma cell line, U-251MG. | lld:pubmed |
| pubmed-article:2477509 | pubmed:affiliation | Department of Neurological Surgery, Kumamoto School of Medicine, Kumamoto University, Japan. | lld:pubmed |
| pubmed-article:2477509 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2477509 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2477509 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
