Linked Life Data

2476222

Source:http://linkedlifedata.com/resource/pubmed/id/2476222

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1989-10-26
pubmed:abstractText
Upon extensive digestion with DNAaseI of placenta chromatin matrix, previously "stripped" from its loosely-bound components by high-salt extraction, a fraction is obtained that contains almost no endogenous DNA methylase activity but whose DNA, if still included in this whole fraction--not if it has been purified to a protein-free condition--is a good substrate for externally added enzyme. This chromatin matrix can even cause a significant stimulation of methylation of single-stranded Micrococcus luteus DNA by placental methylase. In vivo, this phenomenon may have possible counterparts in the existence of highly-methylated regions of chromatin loops that appear to be protected by tightly-bound protein components from digestion of the "stripped loops" with DNAaseI.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0163-4992
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-57
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
A possible role of chromatin and tightly-bound chromatin proteins on enzyme-catalyzed methylation of DNA.
pubmed:affiliation
Dipartimento di Biopatologia Umana, Universitá di Roma, La Sapienza, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't