Linked Life Data

2476023

Source:http://linkedlifedata.com/resource/pubmed/id/2476023

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1989-9-26
pubmed:abstractText
This contribution describes a new microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter as dynamic markers at the light microscopic level. The technique, called nanovid microscopy, is based on the use of contrast enhancement by video techniques and digital image processing. Two applications on living cells are discussed. The first is the receptor-mediated endocytosis of the transferrin receptor, which for the first time can be followed in real time. A second application documents the motion of cell-surface glycoproteins on PTK2-cells. In addition, nanovid microscopy allows the number of gold particles in immunogold staining to be quantified easily. With this tool, the translocation of specific molecules in living cells becomes one of the most enjoyable activities to observe.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0002-9106
pubmed:author
pubmed:issnType
Print
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
282-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Detection of gold probes with video-enhanced contrast microscopy: nanovid microscopy.
pubmed:affiliation
Department of Life Sciences, Janssen Research Foundation, Beerse, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't