Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-10-10
pubmed:abstractText
In order to initiate the study of the functional differences between myosin alkali light chain isoforms and to investigate the mechanisms of their differential expression, we have isolated cDNA clones for two human alkali light chain isoforms. Here we report DNA sequence and RNA blotting analyses that demonstrate that these cDNAs represent transcripts encoding human MLC3F and MLC1Sb. The sequence of the human MLC1Sb cDNA offers the first fully characterized example of a slow-fiber skeletal muscle alkali light chain isoform from any species. The sequence analysis of these two cDNAs allows an examination of evolutionarily conserved features of mammalian alkali light chain genes. Examination of the genomic organization of the human alkali light chain isoform genes revealed that, in contrast with some strains of mice, both are single copy genes. RNA blot analysis conclusively demonstrates that the human skeletal muscle MLC1Sb gene is also expressed in the heart ventricle but not the atria. In addition, we examined the expression of alkali light chain isoforms during the in vitro differentiation of a variety of human and rodent myogenic cells and found striking variation in the pattern of alkali light chain isoform gene expression in different myogenic cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-36
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:2475760-Amino Acid Sequence, pubmed-meshheading:2475760-Animals, pubmed-meshheading:2475760-Base Sequence, pubmed-meshheading:2475760-Biological Evolution, pubmed-meshheading:2475760-Cell Line, pubmed-meshheading:2475760-DNA, pubmed-meshheading:2475760-DNA Probes, pubmed-meshheading:2475760-Humans, pubmed-meshheading:2475760-Isomerism, pubmed-meshheading:2475760-Molecular Sequence Data, pubmed-meshheading:2475760-Muscles, pubmed-meshheading:2475760-Myosin Subfragments, pubmed-meshheading:2475760-Myosins, pubmed-meshheading:2475760-Nucleic Acid Hybridization, pubmed-meshheading:2475760-Peptide Fragments, pubmed-meshheading:2475760-RNA, pubmed-meshheading:2475760-Restriction Mapping, pubmed-meshheading:2475760-Rodentia, pubmed-meshheading:2475760-Sequence Homology, Nucleic Acid
pubmed:year
1989
pubmed:articleTitle
Sequence and expression of human myosin alkali light chain isoforms.
pubmed:affiliation
Department of Medicine, Stanford Medical School, Palo Alto, CA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't