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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2 Pt 1
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pubmed:dateCreated |
1989-9-15
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pubmed:abstractText |
The effects of postnatal dexamethasone treatment in vivo on the synthesis of surfactant protein A (SP-A) were examined at the protein and RNA levels. Rats ranging from 1 day old to adult were injected with 200 micrograms of dexamethasone/kg body wt or with vehicle alone and were killed 24 h after injection. One portion of the lung was metabolically labeled with [35S]methionine, the proteins immunoprecipitated using an antiserum to SP-A, and analyzed electrophoretically. Both newly synthesized intracellular and secreted SP-A levels were increased by dexamethasone, reaching averages of 2.3 and 4.5 times control values, respectively. Another portion of the lung tissue was used for RNA analysis. SP-A mRNA levels were also elevated an average of 1.4 times control values by hormone treatment. Dose-response experiments using 16-day-old pups showed that both total SP-A, as measured by enzyme-linked immunosorbent assay, and total SP-A mRNA levels were elevated with dexamethasone treatment, reaching maximal stimulation at 2 mg. We conclude that postnatal dexamethasone treatment in vivo results in increased levels of both newly synthesized SP-A and SP-A mRNA, suggesting that pretranslational events may in part contribute to this process.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L137-43
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2475036-Animals,
pubmed-meshheading:2475036-Dexamethasone,
pubmed-meshheading:2475036-Dose-Response Relationship, Drug,
pubmed-meshheading:2475036-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2475036-Glycoproteins,
pubmed-meshheading:2475036-Lung,
pubmed-meshheading:2475036-Molecular Weight,
pubmed-meshheading:2475036-Proteolipids,
pubmed-meshheading:2475036-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:2475036-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:2475036-Pulmonary Surfactants,
pubmed-meshheading:2475036-RNA,
pubmed-meshheading:2475036-RNA, Messenger,
pubmed-meshheading:2475036-Rats,
pubmed-meshheading:2475036-Rats, Inbred Strains,
pubmed-meshheading:2475036-Reference Values
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pubmed:year |
1989
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pubmed:articleTitle |
Postnatal stimulation of rat surfactant protein A synthesis by dexamethasone.
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pubmed:affiliation |
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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