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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1989-9-13
|
| pubmed:abstractText |
Glutathione peroxidase (GSH-Px) contains selenium (Se) as selenocysteine in the active site of the enzyme. GSH-Px activities in the cytosol of all guinea-pig tissues examined were extremely low compared with those in mice and rats, while Se concentrations in tissues were almost the same among three animal species. In addition, no GSH-Px mRNA was detectable in any tissues of guinea-pigs, although the guinea-pig had the same copy number (probably a single copy) of the GSH-Px gene in its genomic DNA as that of the mouse and rat, suggesting that the species difference of GSH-Px activity observed in rodents might be due to incapability of gene transcription. On the other hand, feeding of mice with Se-deficient diet for 6 weeks resulted in a remarkable decrease in GSH-Px mRNA as well as GSH-Px activity both in the liver and kidneys. The detailed time-course experiment revealed that the drop in GSH-Px activity preceded the decrease in the mRNA level in Se-depleted mice and the mRNA level recovered rapidly in contrast to the slow rate of increase in the enzyme activity in Se-repleted mice. These results suggested that the alteration in GSH-Px activity in mice subjected to dietary Se manipulation is attributable not only to transcriptional but also to post-transcriptional regulation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
1008
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
301-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2474322-Animals,
pubmed-meshheading:2474322-Binding Sites,
pubmed-meshheading:2474322-Blotting, Southern,
pubmed-meshheading:2474322-Diet,
pubmed-meshheading:2474322-Gene Expression Regulation,
pubmed-meshheading:2474322-Glutathione Peroxidase,
pubmed-meshheading:2474322-Guinea Pigs,
pubmed-meshheading:2474322-Kidney,
pubmed-meshheading:2474322-Liver,
pubmed-meshheading:2474322-Male,
pubmed-meshheading:2474322-Mice,
pubmed-meshheading:2474322-Mice, Inbred ICR,
pubmed-meshheading:2474322-Myocardium,
pubmed-meshheading:2474322-RNA,
pubmed-meshheading:2474322-RNA, Messenger,
pubmed-meshheading:2474322-Rats,
pubmed-meshheading:2474322-Rats, Inbred Strains,
pubmed-meshheading:2474322-Selenium,
pubmed-meshheading:2474322-Species Specificity
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
The regulation of glutathione peroxidase gene expression relevant to species difference and the effects of dietary selenium manipulation.
|
| pubmed:affiliation |
Department of Public Health, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|