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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1989-8-8
|
| pubmed:abstractText |
A total of 21 human fetal pituitaries was collected from spontaneous abortions (11 cases) or prostaglandin (PG)-induced abortions at the second trimester. Pituitaries were homogenized, fractionated by HPLC, and the fractions were tested by specific RIA for alpha-endorphin (EP) (beta-EP 1-16), gamma-EP (beta-EP 1-17), beta-EP (beta-EP 1-31), and their alpha-N-acetylated derivatives. In the fetal pituitaries collected after spontaneous abortion, the contents of alpha-EP (23.4 +/- 7.5 pmol/mg protein, mean +/- SE) and gamma-EP (28.9 +/- 9.7) were similar to those of beta-EP (28.6 +/- 7.4). Both beta-EP/alpha-EP (1.2 +/- 0.3) and beta-EP/gamma-EP (1.1 +/- 0.3) ratios approached unity. Although 35.7 and 30.2% of alpha-EP and gamma-EP were acetylated, acetyl-beta-EP was only 8.4% of the total beta-EP immunoreactivity. In the five cases of PG-induced abortion that were more than 20 wk of pregnancy, the pituitary content of beta-EP (75.9 +/- 21.2) and gamma-EP (26.2 +/- 7.5) were significantly higher than in samples collected after spontaneous abortion (13.3 +/- 8.2 and 5.9 +/- 1.8, respectively, p less than 0.01). On the contrary, neither alpha-EP (31.3 +/- 5.2), acetyl-alpha-EP (0.94 +/- 0.28), acetyl-gamma-EP (0.65 +/- 0.07), acetyl-beta-EP (0.35 +/- 0.05) pituitary contents in PG-induced abortions differed from those measured after spontaneous abortion (alpha-EP: 25.6 +/- 6.6; acetyl-alpha-EP: 0.92 +/- 0.41; acetyl-gamma-EP: 0.82 +/- 0.30; acetyl-beta-EP: 0.96 +/- 0.44).(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0031-3998
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
652-5
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2472594-Acetylation,
pubmed-meshheading:2472594-Endorphins,
pubmed-meshheading:2472594-Fetus,
pubmed-meshheading:2472594-Humans,
pubmed-meshheading:2472594-Pituitary Gland,
pubmed-meshheading:2472594-Radioimmunoassay,
pubmed-meshheading:2472594-alpha-Endorphin,
pubmed-meshheading:2472594-beta-Endorphin,
pubmed-meshheading:2472594-gamma-Endorphin
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Presence of acetylated and shortened endorphins in human fetal pituitary gland.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, University of Modena, School of Medicine, Italy.
|
| pubmed:publicationType |
Journal Article
|