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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-7-21
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pubmed:abstractText |
Rat mast cells and bone marrow-derived mouse mast cells (BMMC) were sensitized with mouse IgE mAb, and permeabilized by ATP to introduce guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) and/or guanosine-5'-O-(2-thiodiphosphate) (GDP beta S) into the cells. After ATP-induced lesions were resealed with Mg2+, the cells were challenged by Ag to determine the effect of the nonhydrolyzable guanosine phosphate on Ag-induced hydrolysis of phosphoinositides and histamine release. Introduction of GTP gamma S into permeabilized rat mast cells or BMMC, followed by exposure of the cells to extracellular Ca2+, resulted in histamine release, but failed to induce hydrolysis of phosphoinositides. It was also found that introduction of GTP gamma S into the cells did not synergistically enhance Ag-induced histamine release. Introduction of GDP beta S into sensitized BMMC inhibited the GTP gamma S-dependent, Ca2+-induced histamine release but failed to inhibit Ag-induced histamine release. The results suggest that GTP gamma S-dependent, Ca2+-induced histamine release and Ag-induced histamine release go through independent biochemical pathways. It was also found that introduction of GTP gamma S or GDP beta S into sensitized BMMC neither enhanced nor inhibited Ag-induced formation of inositol phosphates. These results together with previous findings that pretreatment of BMMC with either pertussis toxin or cholera toxin does not affect Ag-induced hydrolysis of phosphoinositides, indicate that a G protein is not involved in the transduction of IgE-mediated triggering signals to phospholipase C in rodent mast cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Agar,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/dinitrophenyl-human serum albumin...,
http://linkedlifedata.com/resource/pubmed/chemical/guanosine 5'-O-(2-thiodiphosphate)
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
250-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2471737-Agar,
pubmed-meshheading:2471737-Animals,
pubmed-meshheading:2471737-Cell Membrane Permeability,
pubmed-meshheading:2471737-Cells, Cultured,
pubmed-meshheading:2471737-Cyclic AMP,
pubmed-meshheading:2471737-Dinitrophenols,
pubmed-meshheading:2471737-Enzyme Activation,
pubmed-meshheading:2471737-Guanine Nucleotides,
pubmed-meshheading:2471737-Guanosine Diphosphate,
pubmed-meshheading:2471737-Guanosine Triphosphate,
pubmed-meshheading:2471737-Haptens,
pubmed-meshheading:2471737-Histamine Release,
pubmed-meshheading:2471737-Hydrolysis,
pubmed-meshheading:2471737-Immunoglobulin E,
pubmed-meshheading:2471737-Mast Cells,
pubmed-meshheading:2471737-Mice,
pubmed-meshheading:2471737-Mice, Inbred CBA,
pubmed-meshheading:2471737-Phosphatidylinositols,
pubmed-meshheading:2471737-Rats,
pubmed-meshheading:2471737-Rats, Inbred Strains,
pubmed-meshheading:2471737-Serum Albumin,
pubmed-meshheading:2471737-Thionucleotides,
pubmed-meshheading:2471737-Type C Phospholipases
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pubmed:year |
1989
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pubmed:articleTitle |
Effect of nonhydrolyzable guanosine phosphate on IgE-mediated activation of phospholipase C and histamine release from rodent mast cells.
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pubmed:affiliation |
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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