| pubmed-article:2470650 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0380603 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0017374 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2470650 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2470650 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2470650 | pubmed:dateCreated | 1989-6-26 | lld:pubmed |
| pubmed-article:2470650 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2470650 | pubmed:abstractText | Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of beta-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea. | lld:pubmed |
| pubmed-article:2470650 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2470650 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2470650 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2470650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2470650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2470650 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2470650 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2470650 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2470650 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:McCarthyJ EJE | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:GrussPP | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:SchneiderKK | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:RisauWW | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:KnoerzerWW | lld:pubmed |
| pubmed-article:2470650 | pubmed:author | pubmed-author:BinderH PHP | lld:pubmed |
| pubmed-article:2470650 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2470650 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:2470650 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:2470650 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2470650 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2470650 | pubmed:pagination | 21-30 | lld:pubmed |
| pubmed-article:2470650 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2470650 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2470650 | pubmed:articleTitle | Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli. | lld:pubmed |
| pubmed-article:2470650 | pubmed:affiliation | PROGEN Biotechnik GmbH, Heidelberg, F.R.G. | lld:pubmed |
| pubmed-article:2470650 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2470650 | pubmed:publicationType | Comparative Study | lld:pubmed |
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