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rdf:type |
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lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0017374,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0380603,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1989-6-26
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pubmed:databankReference |
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pubmed:abstractText |
Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of beta-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2470650-Amino Acid Sequence,
pubmed-meshheading:2470650-Animals,
pubmed-meshheading:2470650-Antibody Formation,
pubmed-meshheading:2470650-Base Sequence,
pubmed-meshheading:2470650-Blotting, Western,
pubmed-meshheading:2470650-Cattle,
pubmed-meshheading:2470650-Cell Division,
pubmed-meshheading:2470650-Cloning, Molecular,
pubmed-meshheading:2470650-Cornea,
pubmed-meshheading:2470650-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2470650-Escherichia coli,
pubmed-meshheading:2470650-Fibroblast Growth Factors,
pubmed-meshheading:2470650-Gene Expression Regulation,
pubmed-meshheading:2470650-Genes, Synthetic,
pubmed-meshheading:2470650-Genetic Vectors,
pubmed-meshheading:2470650-Humans,
pubmed-meshheading:2470650-Molecular Sequence Data,
pubmed-meshheading:2470650-Oligodeoxyribonucleotides,
pubmed-meshheading:2470650-Phosphorylation,
pubmed-meshheading:2470650-Plasmids,
pubmed-meshheading:2470650-Rabbits,
pubmed-meshheading:2470650-Recombinant Proteins,
pubmed-meshheading:2470650-Staining and Labeling
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pubmed:year |
1989
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pubmed:articleTitle |
Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli.
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pubmed:affiliation |
PROGEN Biotechnik GmbH, Heidelberg, F.R.G.
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pubmed:publicationType |
Journal Article,
Comparative Study
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