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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1989-6-30
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pubmed:abstractText |
The protein bacterio-opsin, complexed with retinal, functions as a light-driven proton pump in the purple membrane of the halophilic archaebacterium, Halobacterium halobium. Bacterio-opsin deficient mutants have been characterized in attempts to elucidate regulation of the gene encoding bacterio-opsin (bop). Analysis of the mutational defect in Bop mutants has revealed the existence of at least two genes that affect bop gene expression and (or) purple membrane formation: (i) the brp gene, located 526 base pairs upstream of the bop gene, is transcribed in the opposite orientation, and (ii) the bat gene, located 1602 base pairs upstream of the bop gene, is transcribed in the same orientation as the brp gene. The bat gene start codon overlaps the stop codon of the brp gene. The bat gene could encode an acidic protein of 73,000 Da (674 amino acids) with a predicted secondary structure typical of a soluble alpha--beta type protein. This type of secondary structure is in contrast to the hydrophobic structure predicted for the putative brp protein. Transcriptional analyses of the wild type, 11 Bop mutants, and a Bop revertant suggest that the bat gene has a more direct role than the brp gene in bop gene expression and is involved in activating bop and brp gene expression.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacteriorhodopsins,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/bacterio-opsin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-4166
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
134-40
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2470479-Archaea,
pubmed-meshheading:2470479-Bacteriorhodopsins,
pubmed-meshheading:2470479-Codon,
pubmed-meshheading:2470479-Gene Expression Regulation,
pubmed-meshheading:2470479-Genes, Bacterial,
pubmed-meshheading:2470479-Halobacterium,
pubmed-meshheading:2470479-Mutation,
pubmed-meshheading:2470479-Protein Conformation,
pubmed-meshheading:2470479-RNA, Bacterial,
pubmed-meshheading:2470479-RNA, Messenger,
pubmed-meshheading:2470479-Restriction Mapping,
pubmed-meshheading:2470479-Transcription, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Regulation of the bacterio-opsin gene of a halophilic archaebacterium.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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