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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-6-19
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pubmed:abstractText |
Antigen-specific sheep T-cell lines have been generated in vitro from peripheral blood mononuclear cells (PBMC). PBMC prepared from antigen-primed animals were cultured in the presence of ovalbumin (OVA) or purified protein derivative (PPD). After 5 days of culture, activated antigen-specific cells were expanded by further culture in the presence of recombinant human interleukin-2 (IL-2). Cell lines generated after two cycles of antigen stimulation followed by expansion with IL-2 show a proliferative response to antigen only in the presence of autologous antigen-presenting cells (APC) and recognize only the antigen used in the original stimulation. An OVA-specific cell line was found to be capable of recognizing a synthetic peptide corresponding to amino acid residues 323-338 of OVA. The cell lines also responded by proliferation in an allogeneic mixed leucocyte reaction (MLR). Cell-surface phenotyping shows that the cell lines comprise both CD4- and CD8-positive cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-13952111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-145543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-2410926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-2413457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-2415139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-3290379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-3308686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-3497349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-3894224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-3894225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-4994445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-5523578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6090574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6166712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6193218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6239901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6298715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6332146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6334313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6443342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6604634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6606011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-6607276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-661981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469643-7047373
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
559-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Antigen presentation in the sheep: generation of antigen-specific T-cell lines.
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pubmed:affiliation |
Department of Veterinary Pathology, University of Edinburgh, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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