Linked Life Data

2469532

Source:http://linkedlifedata.com/resource/pubmed/id/2469532

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-6-16
pubmed:abstractText
The object of this study was to develop a prefixation protein A gold labelling technique for Ureaplasma diversum and to apply this to bovine embryos. Sixteen hour cultures of Ureaplasma diversum strain 2312 were incubated with either specific antiserum or nonimmune serum, followed by exposure to protein A gold and negative staining. The ureaplasmas which were incubated with specific antiserum were labelled with gold particles while those ureaplasmas which were incubated with nonimmune serum were not labelled. Twenty-three unhatched, day 7 bovine embryos were then incubated in either embryo culture medium (ECM) alone, ECM with sterile ureaplasma broth added or ECM with 1.7 X 10(6) colony forming units of Ureaplasma diversum strain 2312 per embryo. After 16 hours, the embryos were washed twice and incubated with either specific antiserum or nonimmune serum. The embryos were then incubated with medium containing protein A gold and examined by electron microscopy. No ureaplasmas were identified on the zona pellucida of the control embryos. Ureaplasmas were identified on the outer surface of the zona pellucida of 13 of the 17 embryos which had been exposed to the organism. Of these, the embryos which were incubated with specific antiserum had labelled ureaplasmas while the embryos which were incubated with nonimmune serum had unlabelled ureaplasmas on the zona pellucida. It was concluded that the protein A gold method was a suitable technique for the identification of ureaplasmas in EM preparations. The presence of ureaplasmas on the outer surface of the bovine zona pellucida following in vitro exposure to the organism was confirmed.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-16725538, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-16725707, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-16725735, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-16726224, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-17422558, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-213601, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-3020081, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-3453263, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-3453276, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-352491, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-3607652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-4130427, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-427710, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-49431, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-6312854, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-7427772, http://linkedlifedata.com/resource/pubmed/commentcorrection/2469532-7427773
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0830-9000
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
172-5
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Protein A gold identification of ureaplasmas on the bovine zona pellucida.
pubmed:affiliation
Department of Pathology, Ontario Veterinary College, University of Guelph.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't