rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1-2
|
pubmed:dateCreated |
1989-6-5
|
pubmed:abstractText |
Simian sarcoma virus (SSV)-infected NIH-3T3 cells (SSV-NIH-3T3), express a homologue of platelet-derived growth factor, (PDGF) a powerful inducer of the c-fos gene. We have used these cells to test the hypothesis that autocrine stimulation by PDGF-like molecules leads to c-fos expression which is functional in the transformed phenotype. We have transfected SSV-NIH-3T3 cells with a c-fos antisense-RNA expression vector, pSVsof, or control plasmids. pSVsof-transfected cells exhibit markedly decreased c-fos mRNA and protein levels, restored density-dependent growth arrest and reduced (three of five clones) tumorigenicity compared to control lines. The results confirm that c-fos cooperates in the transformed phenotype of SSV-NIH-3T3 cells.
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pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0378-1119
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
72
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
253-65
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2468566-Animals,
pubmed-meshheading:2468566-Cell Division,
pubmed-meshheading:2468566-Cell Transformation, Neoplastic,
pubmed-meshheading:2468566-Cells, Cultured,
pubmed-meshheading:2468566-Female,
pubmed-meshheading:2468566-Genetic Vectors,
pubmed-meshheading:2468566-Mice,
pubmed-meshheading:2468566-Mice, Nude,
pubmed-meshheading:2468566-Plasmids,
pubmed-meshheading:2468566-Proto-Oncogene Proteins,
pubmed-meshheading:2468566-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:2468566-Proto-Oncogenes,
pubmed-meshheading:2468566-RNA,
pubmed-meshheading:2468566-RNA, Antisense,
pubmed-meshheading:2468566-RNA, Messenger,
pubmed-meshheading:2468566-Sarcoma Virus, Woolly Monkey,
pubmed-meshheading:2468566-Transcription, Genetic,
pubmed-meshheading:2468566-Transfection
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pubmed:year |
1988
|
pubmed:articleTitle |
Analysis of a transformed cell line using antisense c-fos RNA.
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pubmed:affiliation |
La Jolla Cancer Research Foundation, CA 92037.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|