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pubmed-article:2468165pubmed:abstractTextAn osteoblast calcium channel demonstrated by single channel recordings is associated with calcium antagonist receptor binding sites in osteoblast-like osteosarcoma cells. By using whole cell current recordings we now show that this channel is stimulated by the dihydropyridine calcium agonist drug BAY K 8644. A physiological relevance of these channels is apparent from the stereoselective, potent inhibition of parathyroid hormone-stimulated calcium uptake into osteoblast-like cells in culture by desmethoxyverapamil, a phenylalkylamine calcium antagonist. Secretion by these cells of the bone matrix protein osteocalcin is stimulated by BAY K 8644 and blocked by desmethoxyverapamil and nitrendipine. Evidence for a role of this channel in bone remodeling in intact animals comes from enhanced bone resorption in fetal rat bones observed with BAY K 8644 and stereoselective, potent blockade of resorption by desmethoxyverapamil.lld:pubmed
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pubmed-article:2468165pubmed:articleTitleBone remodeling signaled by a dihydropyridine- and phenylalkylamine-sensitive calcium channel.lld:pubmed
pubmed-article:2468165pubmed:affiliationDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.lld:pubmed
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pubmed-article:2468165pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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