Linked Life Data

2467896

Source:http://linkedlifedata.com/resource/pubmed/id/2467896

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-5-19
pubmed:abstractText
When Chinese Hamster ovary cells were exposed to severe hypoxia they responded with an enhanced synthesis rate of a small group of specific proteins, which we have termed the oxygen regulated proteins (ORPs). The five major ORPs had molecular weights of 33, 80, 100, 150, and 260 kD. ORP260 was the first ORP to be synthesized at enhanced rates after the induction of hypoxia, reaching its maximal synthesis rate after only a few hours. The other ORPs responded slower, but were being synthesized at maximal rates by 8 hr of hypoxia. Upon reoxygenation, the synthesis rates of the ORPs declined rapidly with ORP260 again responding most rapidly. They all reached control levels by 12 hr of recovery. The induction of enhanced synthesis of these proteins required a very severe degree of hypoxia. They were not seen at oxygen levels higher than 0.03%. While enhanced synthesis of the ORPs was turned on and off quickly with induction of, and recovery from hypoxia, the proteins were not degraded at high rates once synthesized. All five major ORPs have apparent half-lives in excess of 24 hr. When cells were exposed to hypoxia for 20 hr and then allowed to recover for 15 hr, a population of cells was generated that had, on average, at least twice the cell volume as aerobic control cells. These large cells also contained greater than 4C DNA content as well as enhanced amounts of RNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0360-3016
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
957-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Enhanced synthesis of specific proteins, RNA, and DNA caused by hypoxia and reoxygenation.
pubmed:affiliation
Department of Biophysics, University of Rochester, NY 14642.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't