| pubmed-article:2467484 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C0221920 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C0439662 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:2467484 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2467484 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2467484 | pubmed:dateCreated | 1989-4-26 | lld:pubmed |
| pubmed-article:2467484 | pubmed:abstractText | A trypsinization technique was optimized for preparation of human epidermal single-cell suspensions from suction blisters. The conditions which provided the highest epidermal cell yield and viability was Sigma trypsin 0.25% (Type II, crude; cat. no. T8128). The incubation time should be 45 min at 37 degrees C with continuous shaking of the epidermal tissue. The use of suction blisters was evaluated for in vivo determination of epidermal thymocyte activating factor (ETAF/Il-1). Trypsinization of epidermis diminished ETAF/Il-1 activity. Dialysis of epidermal homogenates improves determination of ETAF/Il-1-like activity due to removal of low-weight inhibitors. Determination of ETAF/Il-1-like activity in homogenates of suction blisters constitutes a reliable model with acceptable reproducibility. | lld:pubmed |
| pubmed-article:2467484 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2467484 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2467484 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2467484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2467484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2467484 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2467484 | pubmed:issn | 0001-5555 | lld:pubmed |
| pubmed-article:2467484 | pubmed:author | pubmed-author:Thestrup-Pede... | lld:pubmed |
| pubmed-article:2467484 | pubmed:author | pubmed-author:LarsenF GFG | lld:pubmed |
| pubmed-article:2467484 | pubmed:author | pubmed-author:LarsenC GCG | lld:pubmed |
| pubmed-article:2467484 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2467484 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:2467484 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2467484 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2467484 | pubmed:pagination | 474-9 | lld:pubmed |
| pubmed-article:2467484 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:meshHeading | pubmed-meshheading:2467484-... | lld:pubmed |
| pubmed-article:2467484 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2467484 | pubmed:articleTitle | Preparation of human epidermal tissue for functional immune studies. | lld:pubmed |
| pubmed-article:2467484 | pubmed:affiliation | Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark. | lld:pubmed |
| pubmed-article:2467484 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2467484 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2467484 | lld:pubmed |
