Linked Life Data

2467202

Source:http://linkedlifedata.com/resource/pubmed/id/2467202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1989-5-10
pubmed:abstractText
The cell line E2 is a SV40-transformed human fibroblast cell line containing a single integrated copy of the bacterial guanine phosphoribosyl transferase (gpt) gene. Treatment of E2 with ultraviolet light (UV) or ethyl methanesulphonate (EMS) induced the formation of Gpt- derivatives. Several induced derivatives have been isolated, and the structure, expression and revertibility of the gpt gene have been analysed. In the majority of cases the Gpt- phenotype resulted from switching off the gpt gene, in most instances by methylation, but in a few cases by phenotypic switching. Thus mutagenic treatment can result in the inactivation of gene expression in human cells. In a small proportion of Gpt- derivatives the gpt sequences were deleted.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
220
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Mutagenic treatments result in inactivation of expression of a transfected bacterial gene integrated into a human cell line.
pubmed:affiliation
MRC Cell Mutation Unit, University of Sussex, Brighton, Great Britain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't